Normalize, find variable features, scale, and run PCA
Usage
ProcessSeuratObject(
obj,
normalization_method = "LogNormalize",
scale_factor = 10000,
scale_features = NULL,
selection_method = "vst",
verbose = TRUE
)
Arguments
- obj
Seurat object
- normalization_method
Normalization method ("LogNormalize", "CLR", or "RC")
- scale_factor
Scaling factor for normalization
- scale_features
Features to scale
- selection_method
Variable feature selection method ("vst", "mvp", or "disp")
- verbose
Print progress messages